Sexing of remaining vestigial adults:
20 females and 2 males.
Sexing the flies this time was easier, because another distinguishing trait between the two sexes was also used: male fruit flies have dark patches of sex combs on their upper limbs, while female fruit flies only have sparse hairs.
Observations of the other cultures:
Wild type eggs/larva--larvae are still present in the food, and chrysalises on the sides of the culture have still not hatched. Slight discoloration that can be attributed to slime mold growth is also present.
Wild type adults--only one is left alive (a male); more flies may need to be added into this culture.
Vestigial Developing flies--larvae are still digging tunnels in the food, and chrysalises on the sides of the culture have not yet hatched.
Vestigial Adults, previously sexed-- 11 have died, 10 are still alive, and there is no evidence of reproduction within the culture.
White eyed--the culture is still thriving overall, but a large number died in the last 24 hours (no sufficient time to get a count). Larvae are still burrowing in the food source.
**For future reference: When genetic crosses begin, females will have to be counted in the morning. If there are old females mixed in with males, they will need to be killed or released. Newly hatched females will be moved to a separate culture, because they are still virgins for their first 10 hours of life. If they are left to have sex after this time period, they are useless for genetic crosses, as they can store sperm from more than one parent and the specific genes that the offspring will have will not be evident.
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