Day 2: Stock Cultures & Sexing, Part 1

Stock Culture Sexing (In-Class Notes):
--Anestitizing happens in a separate vial
--flip vial over, flies move up (need to be a top surface)
or bang flies to the bottom of the vial
Uncap vial with food and flies, while still banging, overlap with new vial, bang flies into new vial and reseal

--Flys are anestitzed with "Fly Nap" (too much kills them!)
--anestitize within the vial, use stick with cotton on the end.
--1st flies are white-eyes, 1:1 ratio for food and water
--sexing 1st thing, use microscope, separate females & male
How can you tell the sex?
**black anterior ends are males
only males have a sex comb (fringe of black bristles on the forelegs)
tip of the abdomen is elongated and somewhat pointed in females and more rounded in males
abdomen of a female has several segments, whereas the male has only 5

• 5 males, one female in white-eyes 
• 8 male, one female in wild
•   3 male, 2 female  in vestigal 

     To be researched in correlation: life cycles, morphology, care & keeping